UJI AKTIVITAS ANTIOKSIDAN EKSTRAK TELUR KEONG MAS (Pomacea canaliculata) DENGAN METODE DPPH (2,2-DIFENIL-1-PIKRILHIDRAZIL) MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS

Eva Kholifah

doi:10.30591/pjif.v12i3.4817

UJI AKTIVITAS ANTIOKSIDAN EKSTRAK TELUR KEONG MAS (Pomacea canaliculata) DENGAN METODE DPPH (2,2-DIFENIL-1-PIKRILHIDRAZIL) MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS

Eva Kholifah

Abstract

Antioxidant are compound that could donate one or more electron to free radical to inhibit free radical reaction. One of the mollusc that has the potential to be an antioxidant is the egg of the gold snail (Pomacea canaliculata). The purpose of this study was to determine the antioxidant activity of gold snail egg. Testing of antioxidant activity was carried out by the DPPH (2,2-diphenyl-1-picrilhydrazyl) method. Gold snail egg are macerated with a mixture of solvent acetone:methanol (1:8). The extract which had been vaporized, was tested its phytochemical compound with phytochemical screening test, the results of the phytochemical screening of the extract showed positive contain alkaloid, flavonoid, steroid, triterpenoid, saponin, and protein. The gold snail egg extract was then tested for its antioxidant activity quantitatively to obtain IC₅₀ (Inhibitory Concentration) values from the extract using UV-Vis spectrophotometry at a wavelength (λmax) of 515 nm with vitamin C as a positive control. The results of spectrophotometric measurements show that gold snail egg extract has an IC₅₀ value of 4,890 μg / ml and vitamin C has an IC₅₀ value of 2,792 μg / ml. According to the result, it can be concluded that antioxidant activity of golden snail egg extract is lower than vitamin C but still potential as an antioxidant.

Keywords

Pomacea canaliculate; Antioxidant; DPPH

Full Text:

References

Setiawan, F., Yunita, O., & Kurniawan, A. (2018). Uji Aktivitas Antioksidan Ekstrak Etanol Kayu Secang dan FRAP. Fakultas Farmasi Universitas Surabaya, Surabaya. Media Pharmaceutica Indonesiana, 2(2), 82–89.

Murwanti, R., Kholifah, E., Sudarmanto, B. S. A., & Hermawan, A. (2020). Curcumin and its Analogue Targeting β -Catenin and GSK- 3β in Wnt Signalling Pathways : In Vitro and In Silico Study. Departement of Pharmacology and Clinic, Faculty of Pharmacy, Universitas Gadjah Mada. Jurnal Farmasi Dan Teknologi, 13(April), 11–15.

3. Pridatama, Y., & Ilza, M. (2021). Studi Komparatif Metode DPPH Dan Frap Terhadap Aktivitas Antioksidan Ekstrak Telur Keong Mas (Pomacea cannaliculata). Fakultas Perikanan Dan Kelautan Universitas Riau Pekanbaru. 4–11.

Muthia, R., Saputri, R., & Verawati, S. A. (2019). Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Mundar ( Garcinia forbesii King .) Menggunakan Metode DPPH ( 2 , 2-Diphenyl-1- Picrylhydrazi l ). Sekolah Tinggi Ilmu Kesehatan Borneo Lestari, Indonesia. Jurnal Pharmascience, 06(01), 74–82.

Wahdaningsih, S., Budilaksono, W., & Fahrurroji, A. (2015). Uji Aktivitas Antioksidan Fraksi N-Heksana Kulit Buah Naga Merah menggunakan Metode 1,1-Difenil-2-Pikrilhidrazil. Program Studi Farmasi, Fakultas Kedokteran, Universitas Tanjungpura. Jurnal Kesehatan Khatulistiwa, 1(April), 115–136.

Abdullah, A. (2017). Karakterisasi Dan Identifikasi Senyawa Aktif Ekstrak Pigmen Telur Keong Mas. Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Kampus IPB Darmaga, Bogor. IPB Bogor, 20(2006), 286–295.

Maryam, F., Taebe, B., & Toding, D. P. (2020). Pengukuran Parameter Spesifik Dan Non Spesifik Ekstrak Etanol Daun Matoa (Pometia pinnata J.R & G.Forst). Sekolah Tinggi Ilmu Farmasi Makassar. Jurnal Mandala Pharmacon Indonesia, 6(1), 1–12.

Saputri, D. D., & Pertiwi, M. P. (2021). Identifikasi Metabolit Sekunder dan Uji Proksimat Ekstrak Daging Keong Mas ( Pomacea canaliculata L .). Prodi Pendidikan Biologi, FKIP, Universitas Pakuan, Bogor. 22(2), 101– 110.

Harahap, I. S., Halimatussakdiah, & Amna, U. (2021). Skrining Fitokimia Ekstrak Daun Jeruk Lemon ( Citrus limon L .) dari Kota Langsa , Aceh. 1Program Studi Kimia Fakultas Teknik Universitas Samudra. Jurnal Kimia Sains Dan Terapan, 3(April), 19–23.

Dyah Saputri D, Putri Pertiwi M. Identifikasi Metabolit Sekunder dan Uji Proksimat Ekstrak Daging Keong Mas (Pomacea canaliculata L.). Jurnal ILMU DASAR. 2021;22(2):101–10.

Abdullah A, Nurjanah, Seulalae AV. Antioxidant activity of biopigment fractions from golden apple snail eggs (Pomacea canaliculata). IOP Conf Ser: Earth Environ Sci. 2020 Jan 1;404(1):012003.

Boonyapakdee A, Pootangon Y, Laudadio V, Tufarelli V. Astaxanthin extraction from golden apple snail (Pomacea canaliculata) eggs to enhance colours in fancy carp (Cyprinus carpio). Journal of Applied Animal Research. 2015 Jul 3;43(3):291–4.

Heras H, Dreon MS, Ituarte S, Pollero RJ. Egg carotenoproteins in neotropical Ampullariidae (Gastropoda: Arquitaenioglossa). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2007 Jul 1;146(1):158–67.

DOI: https://doi.org/10.30591/pjif.v12i3.4817